Clathrin - Quiz
Clathrin
  • 1. What is the primary role of clathrin in the cytoplasm?
A) Shaping rounded vesicles for intracellular trafficking
B) Synthesizing lipids for the cell membrane
C) Directing protein synthesis
D) Degrading misfolded proteins
  • 2. Where does the selection of cargo by clathrin-coated vesicles occur?
A) Cell membrane, trans-Golgi network, and endosomal compartments
B) Endoplasmic reticulum, ribosomes, and vacuoles
C) Nucleus, mitochondria, and lysosomes
D) Nuclear envelope and peroxisomes
  • 3. What happens to the clathrin coat after a vesicle buds into the cytoplasm?
A) It degrades into amino acids
B) It fuses with the Golgi apparatus
C) It becomes part of the vesicle membrane
D) It rapidly disassembles
  • 4. Which proteins are responsible for the recruitment and self-assembly of clathrin?
A) Microtubule motors
B) Ribosomal proteins
C) Adaptor molecules
D) Histone complexes
  • 5. Which specific protein is mentioned as being used in synaptic vesicle formation?
A) CKAP5
B) TACC3
C) AP180
D) ch-TOG
  • 6. Besides recruiting clathrin, what additional function does Epsin perform?
A) Degrades the clathrin triskelion
B) Helps deform the membrane to aid budding
C) Synthesizes ATP for the budding process
D) Transports vesicles to the nucleus
  • 7. What is the shape formed by clathrin triskelions on the membrane?
A) A polyhedral lattice
B) A spherical droplet
C) A linear filament
D) A hollow cylinder
  • 8. To what does the clathrin triskelion foot bind?
A) Microtubules
B) Lipid bilayer phospholipids
C) The cell membrane directly
D) Adaptor proteins
  • 9. What happens to clathrin-coated vesicle formation during mitosis?
A) It occurs only in the nucleus
B) It increases significantly
C) It continues at a constant rate
D) It is shut down
  • 10. Which two proteins form a complex with clathrin during mitosis?
A) AP180 and Epsin
B) Tubulin and Dynein
C) Actin and Myosin
D) TACC3 and ch-TOG/CKAP5
  • 11. What is the function of the TACC3/ch-TOG/clathrin complex during mitosis?
A) Inhibiting DNA replication
B) Creating the cell wall
C) Regulating cytoplasmic pH
D) Stabilizing kinetochore fibers
  • 12. What is required for the clathrin complex to crosslink microtubules?
A) The presence of Pitstop 2
B) The disassembly of the polyhedral lattice
C) Its trimeric structure
D) Its binding to the Golgi membrane
  • 13. What are Pitstop 1 and Pitstop 2?
A) Microtubule stabilizers
B) Selective clathrin inhibitors
C) Adaptor proteins
D) Growth factors
  • 14. How do Pitstop compounds interfere with cellular invasion?
A) By binding directly to the membrane lipids
B) By blocking endocytic ligand association with the clathrin terminal domain
C) By degrading the spindle apparatus
D) By preventing the synthesis of AP180
  • 15. Which molecule is used to create the microtubule binding surface in the mitotic spindle?
A) The membrane-bound adaptor proteins
B) The amino-terminal domain of clathrin heavy chain and the TACC domain of TACC3
C) The ch-TOG terminal domain only
D) The polyhedral lattice and AP180
  • 16. Who first isolated clathrin in 1976?
A) Barbara Pearse
B) Graeme Mitchison
C) Charles Darwin
D) Robert Hooke
  • 17. What is the shape of a clathrin protein unit?
A) Sphere
B) Cube
C) Cylinder
D) Triskelion
  • 18. Who suggested the name 'clathrin' to Barbara Pearse?
A) Gregor Mendel
B) Louis Pasteur
C) Graeme Mitchison
D) Isaac Newton
  • 19. How many heavy chains are in one clathrin triskelion?
A) Six
B) Two
C) Four
D) Three
  • 20. What is the approximate molecular weight of a clathrin heavy chain?
A) 500 kDa
B) 100 kDa
C) 25 kDa
D) 190 kDa
  • 21. What is the approximate molecular weight of a clathrin light chain?
A) 80 kDa
B) 25 kDa
C) 190 kDa
D) 10 kDa
  • 22. On which human chromosome is the main clathrin heavy chain gene located?
A) Chromosome 13
B) Chromosome 17
C) Chromosome 22
D) Chromosome 1
  • 23. Where is the second clathrin heavy chain gene primarily expressed?
A) Skin
B) Brain
C) Liver
D) Muscle
  • 24. What structure does the N-terminal domain of the clathrin heavy chain form?
A) Random coil
B) Seven-bladed beta-propeller
C) Beta-sheet sandwich
D) Alpha helix bundle
  • 25. To enclose a vesicle, how many pentagons must be present in the lattice?
A) 12
B) 6
C) 36
D) 24
  • 26. What is the smallest clathrin cage commonly imaged called?
A) Mini-coat
B) Micro-cage
C) Triskelion-shell
D) Nano-sphere
  • 27. How many hexagons are in a mini-coat?
A) Two
B) Zero
C) Twelve
D) Five
  • 28. What type of rings are necessary for curved lattice formation?
A) Squares
B) Hexagons
C) Triangles
D) Pentagons
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