How to use a microscope
  • 1. What is the primary function of a microscope?
A) To magnify small objects
B) To dissect specimens
C) To measure distances
D) To illuminate a room
  • 2. Which part of the microscope focuses light onto the specimen?
A) Objective lens
B) Stage
C) Eyepiece
D) Condenser
  • 3. What is the name of the lens you look through?
A) Objective lens
B) Condenser lens
C) Eyepiece
D) Diaphragm
  • 4. Which adjustment knob is used for coarse focusing?
A) Fine adjustment knob
B) Coarse adjustment knob
C) Condenser adjustment knob
D) Stage adjustment knob
  • 5. What is the purpose of the stage clips?
A) To adjust the light intensity
B) To focus the image
C) To hold the slide in place
D) To move the stage
  • 6. Which part controls the amount of light passing through the specimen?
A) Diaphragm
B) Eyepiece
C) Objective lens
D) Coarse focus knob
  • 7. What does 'parfocal' mean in relation to microscope objective lenses?
A) The lenses are all made of glass
B) The image stays in focus when changing objectives
C) The magnification is consistent
D) The image is always perfectly clear
  • 8. How should you carry a microscope?
A) By the eyepiece
B) With one hand on the arm and the other under the base
C) By the objective lenses
D) By the stage
  • 9. What should you use to clean the microscope lenses?
A) Cloth
B) Lens paper
C) Facial tissue
D) Paper towel
  • 10. When using the highest power objective, what adjustment knob should you use?
A) Stage adjustment knob
B) Coarse adjustment knob
C) Fine adjustment knob
D) Condenser adjustment knob
  • 11. What is the total magnification when using a 10x eyepiece and a 40x objective?
A) 1040x
B) 400x
C) 50x
D) 4x
  • 12. What is the first step in preparing a wet mount slide?
A) Place a drop of liquid on the slide
B) Clean the slide
C) Add stain to the specimen
D) Place the coverslip on the slide
  • 13. What is the purpose of a coverslip?
A) To magnify the specimen
B) To hold the slide in place
C) To flatten the specimen and protect the objective lens
D) To add contrast to the specimen
  • 14. Which type of microscope is used to observe living cells in their natural state?
A) Transmission electron microscope
B) Scanning electron microscope
C) Phase contrast microscope
D) Compound microscope
  • 15. What is the purpose of oil immersion?
A) To reduce the amount of light needed
B) To increase resolution at high magnification
C) To stain the specimen
D) To clean the objective lens
  • 16. Before storing the microscope, what objective should be in place?
A) Highest power objective
B) Lowest power objective
C) Any objective
D) Oil immersion objective
  • 17. What does resolution refer to?
A) The depth of focus
B) The brightness of the image
C) The ability to distinguish between two closely spaced objects
D) The magnification power of the microscope
  • 18. What should you do if you can't see anything when looking through the microscope?
A) Increase the magnification
B) Clean the eyepiece
C) Add more stain
D) Check the light source and objective lens position
  • 19. What is the function of the arm of the microscope?
A) Controls the light source
B) Supports the body tube and is used for carrying
C) Supports the stage
D) Holds the objective lenses
  • 20. The base of the microscope...
A) Provides a stable platform for the microscope
B) Adjusts the focus
C) Holds the objective lenses
D) Contains the light source
  • 21. What is the field of view?
A) The brightness of the light source
B) The visible area seen through the eyepiece
C) The objective lens in use
D) The magnification power
  • 22. What is the working distance?
A) The length of the microscope arm
B) The distance between the objective lens and the specimen
C) The field of view diameter
D) The distance between the eyepieces
  • 23. Why is it important to center the specimen before increasing magnification?
A) To prevent the specimen from moving out of the field of view
B) To reduce the amount of light needed
C) To increase the resolution
D) To improve the contrast
  • 24. What is the refractive index?
A) The size of the specimen being observed
B) A measure of how much light bends when it passes from one medium to another
C) The strength of the objective lens
D) The amount of light produced by the light source
  • 25. What is the role of immersion oil in microscopy?
A) To minimize light refraction and improve resolution
B) To clean the objective lens
C) To act as a mounting medium for the specimen
D) To enhance the color of the specimen
  • 26. What is the numerical aperture (NA) of a microscope objective?
A) The field of view diameter
B) The distance between the lens and the specimen
C) A measure of the lens' ability to gather light and resolve fine specimen detail
D) The magnification power of the lens
  • 27. What is Köhler illumination?
A) A technique for optimizing the light path in a microscope to produce a high-quality image
B) A type of stain used in microscopy
C) A type of microscope objective
D) A method for cleaning microscope lenses
  • 28. What type of illumination is used in a darkfield microscope?
A) Polarized light is used to illuminate the specimen
B) Fluorescent light is used to illuminate the specimen
C) Light is scattered by the specimen and enters the objective lens
D) Direct light passes through the specimen
  • 29. What is fluorescence microscopy used for?
A) To perform microsurgery
B) To visualize specific structures or molecules within a cell or tissue
C) To measure the electrical properties of a cell
D) To observe the surface topography of a specimen
  • 30. What is a microtome used for?
A) To measure the size of cells
B) To cut thin sections of tissue for microscopy
C) To mix reagents for staining
D) To sterilize microscope slides
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