How to use a microscope
  • 1. What is the primary function of a microscope?
A) To measure distances
B) To illuminate a room
C) To magnify small objects
D) To dissect specimens
  • 2. Which part of the microscope focuses light onto the specimen?
A) Objective lens
B) Eyepiece
C) Condenser
D) Stage
  • 3. What is the name of the lens you look through?
A) Condenser lens
B) Diaphragm
C) Eyepiece
D) Objective lens
  • 4. Which adjustment knob is used for coarse focusing?
A) Condenser adjustment knob
B) Coarse adjustment knob
C) Fine adjustment knob
D) Stage adjustment knob
  • 5. What is the purpose of the stage clips?
A) To focus the image
B) To adjust the light intensity
C) To hold the slide in place
D) To move the stage
  • 6. Which part controls the amount of light passing through the specimen?
A) Coarse focus knob
B) Objective lens
C) Eyepiece
D) Diaphragm
  • 7. What does 'parfocal' mean in relation to microscope objective lenses?
A) The lenses are all made of glass
B) The image stays in focus when changing objectives
C) The image is always perfectly clear
D) The magnification is consistent
  • 8. How should you carry a microscope?
A) By the eyepiece
B) By the objective lenses
C) With one hand on the arm and the other under the base
D) By the stage
  • 9. What should you use to clean the microscope lenses?
A) Facial tissue
B) Lens paper
C) Paper towel
D) Cloth
  • 10. When using the highest power objective, what adjustment knob should you use?
A) Coarse adjustment knob
B) Fine adjustment knob
C) Condenser adjustment knob
D) Stage adjustment knob
  • 11. What is the total magnification when using a 10x eyepiece and a 40x objective?
A) 50x
B) 4x
C) 400x
D) 1040x
  • 12. What is the first step in preparing a wet mount slide?
A) Add stain to the specimen
B) Place a drop of liquid on the slide
C) Clean the slide
D) Place the coverslip on the slide
  • 13. What is the purpose of a coverslip?
A) To hold the slide in place
B) To add contrast to the specimen
C) To flatten the specimen and protect the objective lens
D) To magnify the specimen
  • 14. Which type of microscope is used to observe living cells in their natural state?
A) Compound microscope
B) Transmission electron microscope
C) Scanning electron microscope
D) Phase contrast microscope
  • 15. What is the purpose of oil immersion?
A) To reduce the amount of light needed
B) To stain the specimen
C) To clean the objective lens
D) To increase resolution at high magnification
  • 16. Before storing the microscope, what objective should be in place?
A) Any objective
B) Oil immersion objective
C) Lowest power objective
D) Highest power objective
  • 17. What does resolution refer to?
A) The ability to distinguish between two closely spaced objects
B) The depth of focus
C) The brightness of the image
D) The magnification power of the microscope
  • 18. What should you do if you can't see anything when looking through the microscope?
A) Increase the magnification
B) Add more stain
C) Clean the eyepiece
D) Check the light source and objective lens position
  • 19. What is the function of the arm of the microscope?
A) Supports the body tube and is used for carrying
B) Controls the light source
C) Holds the objective lenses
D) Supports the stage
  • 20. The base of the microscope...
A) Adjusts the focus
B) Holds the objective lenses
C) Contains the light source
D) Provides a stable platform for the microscope
  • 21. What is the field of view?
A) The brightness of the light source
B) The visible area seen through the eyepiece
C) The magnification power
D) The objective lens in use
  • 22. What is the working distance?
A) The distance between the eyepieces
B) The length of the microscope arm
C) The distance between the objective lens and the specimen
D) The field of view diameter
  • 23. Why is it important to center the specimen before increasing magnification?
A) To reduce the amount of light needed
B) To prevent the specimen from moving out of the field of view
C) To improve the contrast
D) To increase the resolution
  • 24. What is the refractive index?
A) The strength of the objective lens
B) A measure of how much light bends when it passes from one medium to another
C) The amount of light produced by the light source
D) The size of the specimen being observed
  • 25. What is the role of immersion oil in microscopy?
A) To clean the objective lens
B) To enhance the color of the specimen
C) To minimize light refraction and improve resolution
D) To act as a mounting medium for the specimen
  • 26. What is the numerical aperture (NA) of a microscope objective?
A) The magnification power of the lens
B) The field of view diameter
C) The distance between the lens and the specimen
D) A measure of the lens' ability to gather light and resolve fine specimen detail
  • 27. What is Köhler illumination?
A) A type of stain used in microscopy
B) A type of microscope objective
C) A technique for optimizing the light path in a microscope to produce a high-quality image
D) A method for cleaning microscope lenses
  • 28. What type of illumination is used in a darkfield microscope?
A) Polarized light is used to illuminate the specimen
B) Light is scattered by the specimen and enters the objective lens
C) Fluorescent light is used to illuminate the specimen
D) Direct light passes through the specimen
  • 29. What is fluorescence microscopy used for?
A) To measure the electrical properties of a cell
B) To perform microsurgery
C) To observe the surface topography of a specimen
D) To visualize specific structures or molecules within a cell or tissue
  • 30. What is a microtome used for?
A) To measure the size of cells
B) To cut thin sections of tissue for microscopy
C) To sterilize microscope slides
D) To mix reagents for staining
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