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Supercritical Fluid Chromatography
  • 1. Which of the following is commonly used as the mobile phase in SFC?
A) Carbon dioxide
B) Water
C) Methanol
D) Acetone
  • 2. What state is the mobile phase in SFC?
A) Liquid
B) Supercritical
C) Solid
D) Gas
  • 3. What is the mechanism of separation in SFC based on?
A) Differences in boiling point
B) Differences in conductivity
C) Differences in molecular weight
D) Differences in solubility in the mobile phase
  • 4. What is the purpose of using a back pressure regulator in SFC?
A) Maintain supercritical conditions
B) Avoid solvent contamination
C) Enhance detector sensitivity
D) Increase column temperature
  • 5. What advantage does SFC offer over traditional HPLC?
A) Higher resolution
B) Faster separation
C) Lower equipment cost
D) Limited application range
  • 6. What role does co-solvent play in SFC separations?
A) Stabilizes column efficiency
B) Improves stationary phase selectivity
C) Reduces detector interference
D) Enhances analyte solubility
  • 7. Which term describes the region where the mobile phase in SFC exhibits properties of both a liquid and a gas?
A) Transition zone
B) Supercritical zone
C) Near-critical region
D) Critical point
  • 8. At supercritical conditions, the density of carbon dioxide is similar to that of which phase?
A) Liquid
B) Gas
C) Solid
D) Aqueous
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