RNA-binding protein - Quiz
RNA binding protein
  • 1. What does the abbreviation RBP stand for?
A) Ribonucleoprotein binding protein
B) RNA-binding peptide
C) Ribosome-binding protein
D) RNA-binding protein
  • 2. Where are most RBPs located within the cell?
A) Only in the mitochondria
B) Only in the cytoplasm
C) Cytoplasmic and nuclear
D) Only in the nucleus
  • 3. What are heterogeneous ribonucleoprotein particles (hnRNPs)?
A) Proteins that bind to DNA
B) Proteins that bind to lipids
C) Complexes of protein and mature mRNA
D) Complexes of protein and pre-mRNA in the nucleus
  • 4. Which of the following is a structural motif found in RBPs?
A) Protein recognition motif
B) RNA recognition motif (RRM)
C) Lipid recognition motif
D) DNA recognition motif
  • 5. What is a major role of RBPs in post-transcriptional control?
A) Replication
B) Translation
C) Splicing
D) Transcription
  • 6. How many genes encoding RBPs are there in humans according to EuRBPDB?
A) 1500
B) 5000
C) 2961
D) 10000
  • 7. What is the role of RBPs in RNA metabolism?
A) Only regulate RNA biogenesis
B) Only regulate RNA stability
C) Regulate every aspect including biogenesis, maturation, transport, localization, and stability
D) Only regulate RNA transport
  • 8. What type of RNAs do RBPs bind to besides mRNA?
A) Only DNA
B) Functional non-coding RNAs
C) Only proteins
D) Only mRNA
  • 9. Which of the following is a type of non-coding RNA that functions as a ribonucleoprotein complex?
A) MicroRNAs
B) tRNA
C) rRNA
D) mRNA
  • 10. What is the significance of the diversity of RBPs in eukaryotic cells?
A) Prevents RNA exons from being utilized
B) Reduces the number of introns
C) Limits RNA exons to a single arrangement
D) Allows utilization of RNA exons in various arrangements
  • 11. How do RBPs recognize specific RNAs?
A) Through rearrangement of DNA sequences
B) Through rearrangement of basic domains
C) Through rearrangement of protein sequences
D) Through rearrangement of lipid sequences
  • 12. What is the role of RNA-binding proteins in gene expression?
A) Replication regulation
B) Translation regulation
C) Transcriptional regulation
D) Post-transcriptional regulation
  • 13. Which of the following is a function of RBPs in post-transcriptional control?
A) Protein stabilization
B) DNA stabilization
C) mRNA stabilization
D) Lipid stabilization
  • 14. What is the role of RBPs in mRNA localization?
A) They play a major role
B) They have no role
C) They inhibit mRNA localization
D) They degrade mRNA
  • 15. What is the relationship between the diversity of RBPs and the number of introns?
A) Diversity of RBPs increased with the increase in the number of introns
B) Diversity of RBPs prevents the increase in the number of introns
C) Diversity of RBPs decreased with the increase in the number of introns
D) Diversity of RBPs is unrelated to the number of introns
  • 16. What is the role of RBPs in the formation of ribonucleoprotein complexes?
A) They bind to RNA to form these complexes
B) They bind to proteins to form these complexes
C) They bind to DNA to form these complexes
D) They bind to lipids to form these complexes
  • 17. Which of the following is a type of small nuclear RNA?
A) Spliceosomal small nuclear RNAs (snRNA)
B) tRNA
C) rRNA
D) mRNA
  • 18. What is the role of RBPs in RNA biogenesis?
A) They degrade RNA during biogenesis
B) They inhibit RNA biogenesis
C) They have no role in RNA biogenesis
D) They regulate RNA biogenesis
  • 19. What is the role of RBPs in RNA maturation?
A) They degrade RNA during maturation
B) They regulate RNA maturation
C) They inhibit RNA maturation
D) They have no role in RNA maturation
  • 20. Which RNA-binding protein is known for controlling alternative splicing by recognizing specific sequences?
A) NOVA1
B) U1 snRNP
C) U2AF snRNP
D) ADAR
  • 21. What sequence does NOVA1 recognize in RNA for alternative splicing?
A) AUGG
B) GCGC
C) YCAY where Y indicates pyrimidine (U or C).
D) UUUU
  • 22. Where does polyadenylation typically occur in relation to the AAUAAA sequence?
A) About 20 bases downstream.
B) At the 5' untranslated region.
C) Directly at the AAUAAA sequence.
D) About 50 bases upstream.
  • 23. Which protein complex is necessary for the polyadenylation process?
A) TAP/NXF1:p15.
B) CPSF.
C) FMRP.
D) Aly/REF.
  • 24. Which protein is stimulated by CPSF and poly(A)-binding protein to function properly?
A) Poly(A) polymerase.
B) TAP/NXF1:p15.
C) CPSF.
D) Aly/REF.
  • 25. What is the key player in mRNA export?
A) CPSF.
B) Poly(A) polymerase.
C) TAP/NXF1:p15 heterodimer.
D) ZBP1.
  • 26. Which protein recruits TAP by interacting with mRNA?
A) ZBP1.
B) Aly/REF.
C) CPSF.
D) Poly(A) polymerase.
  • 27. Which RBP binds to beta-actin mRNA and localizes it to the lamella region?
A) ZBP1.
B) Aly/REF.
C) FMRP.
D) CPSF.
  • 28. What role does ZBP1 play in the regulation of beta-actin mRNA?
A) It modifies beta-actin mRNA sequences.
B) It degrades beta-actin mRNA.
C) It represses translation by blocking translation initiation.
D) It enhances translation by facilitating ribosome binding.
  • 29. When do some RNA-binding proteins remain bound to RNA?
A) Only during RNA splicing.
B) From transcription until degradation.
C) Only during translation.
D) Only during DNA replication.
  • 30. Which method is used to identify direct RNA binding sites of RNA-binding proteins?
A) Cross-linking immunoprecipitation (CLIP).
B) Gel electrophoresis.
C) Polymerase chain reaction (PCR).
D) Southern blotting.
  • 31. How many amino acids typically make up the RNA-recognition motif (RRM)?
A) 75–85 amino acids
B) 50–60 amino acids
C) 30–40 amino acids
D) 100–110 amino acids
  • 32. What structural elements form the RNA-recognition motif (RRM)?
A) Two α-helices against a three-stranded β-sheet
B) Three α-helices and a two-stranded β-sheet
C) A four-stranded β-sheet against two α-helices
D) A single α-helix and a five-stranded β-sheet
  • 33. How many structures of the RRM have been identified through NMR spectroscopy and X-ray crystallography?
A) Ten
B) Fifteen
C) Twenty
D) Five
  • 34. What is the size range of the double-stranded RNA-binding motif (dsRM)?
A) 50–60 amino acids
B) 100–110 amino acids
C) 70–75 amino acids
D) 30–40 amino acids
  • 35. Which RNA-binding protein is involved in regulating tissue-specific alternative splicing during somatic development?
A) ZBP1
B) MEC-8
C) Sam68
D) CIRBP
  • 36. Which RNA-binding protein specifically binds to the UUGUUGUGUUGU mRNA stretch?
A) UNC-75
B) Sam68
C) Slr1
D) CIRBP
  • 37. In which cells does UNC-75 activate splicing between exon 7a and exon 8?
A) Candida albicans cells
B) Neuronal cells
C) Hypodermis cells
D) Excretory canal cells
  • 38. Which RNA-binding protein is involved in controlling polarized growth in Candida albicans?
A) Slr1
B) UNC-75
C) Sam68
D) CIRBP
  • 39. Which RNA-binding protein is associated with fragile X-associated tremor/ataxia syndrome when lost?
A) CIRBP
B) Sam68
C) UNC-75
D) Slr1
  • 40. Which RNA-binding protein is involved in controlling the cellular response to hypoxia?
A) Sam68
B) CIRBP
C) UNC-75
D) Slr1
  • 41. Which RNA-binding protein is differentially expressed in lung cancer due to CNV losses?
A) KHDRBS2
B) Sam68
C) UNC-75
D) CIRBP
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