- 1. Virology is the branch of science that focuses on the study of viruses and viral diseases. Viruses are small infectious agents that can only replicate inside living cells of other organisms. Virologists investigate the structure, classification, evolution, and interactions of viruses, as well as the mechanisms by which viruses cause disease in their hosts. Understanding virology is crucial in the development of treatments and vaccines for viral infections, and in preventing the spread of viral diseases. The field of virology is constantly evolving as new viruses emerge and existing viruses mutate, posing ongoing challenges to public health and medicine.
What is a virus?
A) A type of bacteria
B) A plant cell
C) A small infectious agent that replicates only inside the living cells of other organisms.
D) A single-celled organism
- 2. What is the protein coat surrounding the genetic material of a virus called?
A) Nucleocapsid
B) Capsid
C) Glycoprotein
D) Envelope
- 3. Which of the following is a method of viral entry into a host cell?
A) Endocytosis
B) Photosynthesis
C) Mitosis
D) Aerobic respiration
- 4. What is the outermost layer of some viruses that is composed of lipids and proteins?
A) Nucleocapsid
B) Capsid
C) Envelope
D) Spike protein
- 5. What is the name of the enzyme that retroviruses use to convert RNA into DNA?
A) DNA polymerase
B) Ligase
C) Reverse transcriptase
D) RNA polymerase
- 6. What is antigenic drift in viruses?
A) Antibody production against viral proteins
B) Sudden change in host specificity
C) Gradual accumulation of mutations in viral genes over time
D) Rapid mutation leading to new strains
- 7. Which type of genetic material is found in retroviruses?
A) RNA
B) Protein
C) Lipid
D) DNA
- 8. Which viral disease has been completely eradicated through vaccination?
A) Smallpox
B) HIV/AIDS
C) Ebola virus
D) Zika virus
- 9. Which of the following is a common symptom of viral infections?
A) High blood pressure
B) Yellowish skin
C) Fever
D) Broken bones
- 10. Who is acknowledged for officially beginning the field of virology as a distinct discipline?
A) Martinus Beijerinck
B) Rosalind Franklin
C) Frederick Twort
D) Ernest William Goodpasture
- 11. What term did Martinus Beijerinck use to describe the novel pathogen causing tobacco mosaic disease?
A) Poliovirus
B) Bacteriophage
C) Vaccinia virus
D) Contagium vivum fluidum
- 12. Who first discovered the full structure of the tobacco mosaic virus?
A) Ernst Ruska
B) Wendell Meredith Stanley
C) Heinz Fraenkel-Conrat
D) Rosalind Franklin
- 13. In what year did Dmitri Ivanovsky use the Chamberland filter to study tobacco mosaic virus?
A) 1884
B) 1906
C) 1892
D) 1913
- 14. Who independently discovered bacteriophages in 1917?
A) Paul Frosch
B) Félix d'Herelle
C) Frederick Twort
D) Dmitri Ivanovsky
- 15. Which virus was the first to be crystallized, allowing its structure to be elucidated in detail?
A) Hepatitis B virus
B) Tobacco mosaic virus
C) Influenza virus
D) Poliovirus
- 16. Who were the first to grow poliovirus in cultured cells from aborted human embryonic tissue?
A) John Franklin Enders, Thomas Weller, and Frederick Robbins
B) Ross Granville Harrison, E. Steinhardt, C. Israeli
C) Ernest William Goodpasture and Alice Miles Woodruff
D) H. B. Maitland and M. C. Maitland
- 17. Who invented the electron microscope in 1931?
A) Frederick Twort and Félix d'Herelle
B) John Franklin Enders and Thomas Weller
C) Wendell Meredith Stanley and Rosalind Franklin
D) Ernst Ruska and Max Knoll
- 18. In which year did Wendell Meredith Stanley find that the tobacco mosaic virus was mostly made of protein?
A) 1957
B) 1941
C) 1963
D) 1935
- 19. Who first isolated HIV, a retrovirus, in 1983?
A) Michael Houghton's team at Chiron Corporation
B) Luc Montagnier's team at the Pasteur Institute
C) Baruch Blumberg
D) Howard Temin
- 20. Which virus was discovered by Baruch Blumberg in 1963?
A) Human immunodeficiency virus (HIV)
B) Equine arterivirus
C) Bovine viral diarrhoea
D) Hepatitis B virus
- 21. Who described the first retrovirus in 1965?
A) Baruch Blumberg
B) Luc Montagnier
C) Michael Houghton
D) Howard Temin
- 22. Which scientist's work enabled the production of an effective polio vaccine?
A) Jonas Salk
B) Hilary Koprowski
C) Frederick Robbins
D) John Franklin Enders
- 23. Which viruses were among the first to be discovered and are useful for studying virus biology?
A) Bacteriophages
B) Influenza viruses
C) Rotaviruses
D) Coronaviruses
- 24. What suffix is used for genera in the ICTV classification system?
A) -viricetes
B) -virales
C) -viridae
D) -virus
- 25. What solution is commonly used in buoyant density centrifugation to form a gradient?
A) Coomasie blue
B) Polyacrylamide gel
C) Ethidium bromide
D) Caesium chloride
- 26. What type of genome do Group III viruses have in the Baltimore classification?
A) (+)ssRNA
B) dsRNA
C) ssDNA
D) dsDNA-RT
- 27. Which type of virus genome is associated with Group VI in the Baltimore classification?
A) (−)ssRNA viruses
B) dsRNA viruses
C) dsDNA viruses
D) ssRNA-RT viruses
- 28. Which technique is not as common in nature but is useful in laboratories to study viral genes?
A) Cloning
B) Recombination
C) RNA silencing
D) Phage display techniques
- 29. Which suffix is used for suborders in the ICTV classification system?
A) -virineae
B) -virus
C) -virales
D) -viricetes
- 30. Who grew influenza and several other viruses in fertilized chicken eggs in 1931?
A) Ernest William Goodpasture and Alice Miles Woodruff
B) H. B. Maitland and M. C. Maitland
C) E. Steinhardt, C. Israeli, and R.A. Lambert
D) Ross Granville Harrison
- 31. Which method is used when virus suspensions remain contaminated after differential centrifugation?
A) Electrophoresis
B) Low speed centrifugation
C) Buoyant density centrifugation
D) Sanger sequencing
- 32. What technique was used to quickly manufacture tests at the start of the COVID-19 pandemic?
A) Recombination
B) RNA silencing
C) Phage display techniques
D) Cloning
- 33. Which staining method is used for visualizing nucleic acids in electrophoresis?
A) Radioactive labeling
B) PHYLIP software
C) Coomasie blue
D) Ethidium bromide
- 34. Which suffix is used for virus families in the ICTV classification system?
A) -virales
B) -viridae
C) -virus
D) -viricetes
- 35. How many realms of viruses have been defined by the ICTV as of 2021?
A) 6
B) 10
C) 17
D) 39
- 36. In the Baltimore classification, which group includes viruses with double-stranded DNA genomes?
A) Group II
B) Group I
C) Group III
D) Group IV
- 37. How are results of the FFA expressed?
A) As infectious dose 50%.
B) As viral load in copies per milliliter.
C) As focus forming units per milliliter (FFU/mL).
D) As plaque forming units per milliliter.
- 38. What are bacteriophages informally called when they reproduce in bacteria?
A) Plasmids
B) Viruses
C) Recombinants
D) Phages
- 39. In what year was the International Committee on Taxonomy of Viruses (ICTV) formed?
A) 1980.
B) 1999.
C) 1975.
D) 1966.
- 40. Who demonstrated that purified tobacco mosaic virus RNA and protein coat can assemble to form functional viruses?
A) Wendell Meredith Stanley
B) Bernal and Fankuchen
C) Heinz Fraenkel-Conrat and Robley Williams
D) Ernst Ruska and Max Knoll
- 41. What type of centrifuge is typically used to concentrate viruses?
A) Low speed centrifuges with a top speed of 10,000 rpm
B) Centrifuges used for blood separation
C) Ordinary laboratory microcentrifuges
D) Ultracentrifuges with a top speed around 100,000 rpm
- 42. In what year was the bacteriophage first discovered by Frederick Twort?
A) 1915
B) 1957
C) 1946
D) 1898
- 43. What classification system is used to supplement the traditional hierarchy for viruses?
A) The Lwoff-Horne-Tournier system.
B) The ICTV 15-rank classification system.
C) The Baltimore classification system.
D) The Linnaean hierarchical system.
- 44. What is a common type of cloning vector used in virology?
A) Bacteriophages
B) Recombinant DNA
C) Laboratory modified plasmids
D) RNA molecules
- 45. Which virus was first grown on a large scale for vaccine production in the 1950s?
A) Poliovirus
B) Hepatitis B virus
C) Tobacco mosaic virus
D) Influenza virus
- 46. Which group in the Baltimore classification includes viruses with double-stranded DNA that use reverse transcriptase?
A) Group II
B) Group VII
C) Group III
D) Group I
- 47. Which group in the Baltimore classification includes viruses with single-stranded RNA that is positive-sense?
A) Group VII
B) Group VI
C) Group IV
D) Group V
- 48. Which software is commonly used to draw phylogenetic trees?
A) Next-generation sequencing (NGS)
B) Coomasie blue
C) PHYLIP
D) Ethidium bromide
- 49. In the Baltimore classification, which group includes viruses with single-stranded RNA that is negative-sense?
A) Group V
B) Group VI
C) Group VII
D) Group IV
- 50. Which suffix is used for classes in the ICTV classification system?
A) -virales
B) -viridae
C) -virus
D) -viricetes
- 51. As of 2021, how many species of viruses have been defined by the ICTV?
A) 65
B) 39
C) 233
D) 10,434
- 52. How many orders of viruses have been defined by the ICTV as of 2021?
A) 39
B) 233
C) 168
D) 65
- 53. What technique uses bacteriophages for screening protein DNA sequences?
A) Cloning
B) Recombination
C) Phage display techniques
D) RNA silencing
- 54. What is the process called when genes from different parents are switched in viruses with segmented genomes?
A) Reassortment
B) Cloning
C) Recombination
D) RNA silencing
- 55. What was commonly used before PCR to confirm viral infections?
A) Microfluidic tests.
B) Immunofluorescence.
C) Complement fixation tests.
D) Lateral flow tests.
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