Growth medium - Quiz
Growth medium
  • 1. What is a minimal medium defined as?
A) A medium containing only amino acids
B) A medium that forbids all bacterial growth
C) A complex medium used for human cells
D) A defined medium with just enough ingredients to support growth
  • 2. What is typically omitted from minimal media?
A) Water
B) Amino acids
C) Carbon sources
D) Salts
  • 3. What type of microorganisms are often grown using minimal media?
A) Pathogenic viruses
B) Only Gram-negative bacteria
C) Wild-type
D) Mutated animal cells
  • 4. What is an example of a carbon source in minimal media?
A) Ampicillin
B) Tetracycline
C) Neomycin
D) Glucose
  • 5. What do the salts in minimal media provide?
A) Proline and histidine
B) Antibiotics and dyes
C) Magnesium, nitrogen, phosphorus, and sulfur
D) Glucose and sucrose
  • 6. What are supplementary minimal media used for?
A) Killing all bacteria in a sample
B) Growing wild-type microorganisms exclusively
C) Culturing specific lines of auxotrophic recombinants
D) Replacing water in the growth process
  • 7. What is the purpose of selective media?
A) Preventing all bacterial metabolism
B) Measuring the concentration of water
C) Growth of only selected microorganisms
D) Growing all types of bacteria equally
  • 8. Which antibiotics are mentioned as agents used to create selective media?
A) Penicillin and aspirin
B) Ampicillin or tetracycline
C) Nalidixic acid and colistin
D) Gancyclovir and neomycin
  • 9. What term is used for a gene that allows a cell to grow in a selective medium?
A) Recombinant
B) Exconjugant
C) Auxotroph
D) Marker
  • 10. What is used in selective media for eukaryotic cells to select transfected cells?
A) Glucose
B) Proline
C) Neomycin
D) Histidine
  • 11. What is the exception to the rule that markers allow cell growth in selective media?
A) Tetracycline
B) Ampicillin
C) Neomycin
D) Gancyclovir
  • 12. Which substance in Eosin methylene blue makes it toxic for Gram-positive bacteria?
A) Glucose
B) Salt
C) Dyes
D) Agar
  • 13. What characteristic of YM and MEA agar deters bacterial growth?
A) High salt concentration
B) Low pH
C) Lack of water
D) High antibiotic content
  • 14. What is MacConkey agar used for?
A) Gram-negative bacteria
B) Fungi only
C) Gram-positive bacteria
D) Virus isolation
  • 15. What does HIS-selective medium lack?
A) Proline
B) Salts
C) Glucose
D) Histidine
  • 16. What is Mannitol salt agar differential for?
A) Legionella
B) Gram-negative bacteria
C) Staphylococci
D) Mannitol
  • 17. Which medium is selective for Legionella pneumophila?
A) Baird-Parker agar
B) Sabouraud agar
C) MacConkey agar
D) Buffered charcoal yeast extract agar
  • 18. What is the primary use of Baird-Parker agar?
A) Moulds and yeasts
B) Gram-positive staphylococci
C) Legionella pneumophila
D) Ectomycorrhizal fungi
  • 19. What contributes to Sabouraud agar's selectivity for fungi?
A) Presence of antibiotics only
B) Low pH and high glucose
C) High salt concentration
D) Lack of nitrogen
  • 20. What is the purpose of dichloran and rose Bengal in DRBC agar?
A) To increase the pH of the medium
B) To restrict the growth of mould colonies
C) To kill all yeast cells
D) To provide nitrogen to the fungi
  • 21. Which media are used for ectomycorrhizal fungi?
A) MacConkey and Hektoen
B) Baird-Parker and DRBC
C) MMN and BAF medium
D) CNA and Sabouraud
  • 22. What is the function of nalidixic acid and colistin in CNA agar?
A) To promote the growth of mould
B) To inhibit Gram-negative organisms
C) To act as a carbon source
D) To kill all Gram-positive bacteria
  • 23. What is a defining characteristic of a chemically defined medium?
A) It contains animal tissue
B) The exact composition is unknown
C) It contains yeast extract
D) All chemicals used are known
  • 24. Which ingredient is strictly absent from a defined medium?
A) Glucose
B) Water
C) Plant tissue
D) Salts
  • 25. What substance is required in an undefined medium alongside salts and carbon sources?
A) Plant hormones
B) Antibiotics
C) Animal blood
D) Water
  • 26. Where are bacteria typically kept in long-term laboratory settings using general culture media?
A) Laboratory culture collections
B) Sterile air chambers
C) Natural soil samples
D) Defined synthetic environments
  • 27. What is a growth medium designed to support?
A) Cell proliferation
B) Cell apoptosis
C) Cell desiccation
D) Cell mutation
  • 28. Which plant is specifically mentioned as being grown in growth media?
A) Zea mays
B) Physcomitrella patens
C) Arabidopsis thaliana
D) Oryza sativa
  • 29. What type of organisms are viruses?
A) Autotrophic microbes
B) Facultative anaerobes
C) Obligate intracellular parasites
D) Extracellular saprophytes
  • 30. Which bacterial genus is capable of decomposing agar?
A) Streptococcus
B) Escherichia
C) Bacillus
D) Staphylococcus
  • 31. What do bacteria grown in liquid cultures often form?
A) Biofilm mats
B) Crystalline structures
C) Sedimentary layers
D) Colloidal suspensions
  • 32. What must be added to animal cell media to support their growth?
A) Agarose
B) Ammonium salts
C) Blood serum
D) Glucose only
  • 33. What is the liquid used to make beer that serves as a growth medium for yeast?
A) Wort
B) Mash
C) Tincture
D) Must
  • 34. What is produced when yeast grows in wort under anaerobic conditions?
A) Hydrogen gas
B) Alcohol
C) Lactic acid
D) Acetic acid
  • 35. What term describes organisms that require specialized environments due to complex needs?
A) Ubiquitous
B) Opportunistic
C) Fastidious
D) Autotrophic
  • 36. What is the primary function of differential media?
A) Supporting fastidious organisms only
B) Preventing microbial multiplication
C) Distinguishing one microorganism type from another
D) Storing specimens for long periods
  • 37. Which of the following is an example of an indicator used in differential media?
A) Sodium chloride
B) Neutral red
C) Glycerol
D) Charcoal
  • 38. What does blood agar become in the presence of beta-hemolytic organisms?
A) Transparent
B) Red
C) Brown
D) Opaque
  • 39. What color do colonies of Streptococcus agalactiae appear on Granada medium?
A) Brown
B) Blue
C) Transparent
D) Red
  • 40. X-gal plates are used to differentiate what?
A) Enteric bacilli
B) Lac operon mutants
C) Strict anaerobes
D) Gonococci
  • 41. Transport media for anaerobes must be free of what?
A) Lactose
B) Glycerol
C) Molecular oxygen
D) Charcoal
  • 42. What do enriched media contain to support fastidious organisms?
A) Extra nutrients
B) Carbon-free buffers
C) Bacterial inhibitors
D) Reducing agents
  • 43. At what temperature range is blood treated to create chocolate agar?
A) 60–65 °C
B) 80–85 °C
C) 20–25 °C
D) 40–45 °C
  • 44. Eosin methylene blue is differential for what?
A) Lactose fermentation
B) Beta-hemolysis
C) Mannitol fermentation
D) Anaerobic growth
  • 45. Thioglycolate broth is designed for which type of organisms?
A) Strict anaerobes
B) Enteric bacilli
C) Fastidious aerobes
D) Lac operon mutants
  • 46. What should transport media maintain in specimens?
A) Viability
B) Increased metabolic rate
C) High concentration
D) Active fermentation
  • 47. What is a potential risk associated with using serum-containing media?
A) Contamination issues
B) Insufficient glucose levels
C) Lack of growth factors
D) Inability to support cell growth
  • 48. Which of the following media types is known for having a standardized formulation?
A) Fetal bovine serum
B) Serum-free media
C) Serum-containing media
D) Animal-derived medium
  • 49. Why is the choice of media important for protein expression?
A) It determines the genetic sequence of the protein
B) It prevents the production of proteins
C) It influences therapeutic characteristics like glycosylation
D) It eliminates the need for purification
  • 50. What are Plasmax and HPLM examples of?
A) Media used only for bacterial cultures
B) Chemically undefined media
C) Media that represent physiological nutrient levels
D) Serum-containing growth media
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