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Clathrin - Quiz
Contributed by: Blake
  • 1. What is the primary role of clathrin in the cytoplasm?
A) Synthesizing lipids for the cell membrane
B) Degrading misfolded proteins
C) Directing protein synthesis
D) Shaping rounded vesicles for intracellular trafficking
  • 2. Where does the selection of cargo by clathrin-coated vesicles occur?
A) Nucleus, mitochondria, and lysosomes
B) Cell membrane, trans-Golgi network, and endosomal compartments
C) Nuclear envelope and peroxisomes
D) Endoplasmic reticulum, ribosomes, and vacuoles
  • 3. What happens to the clathrin coat after a vesicle buds into the cytoplasm?
A) It becomes part of the vesicle membrane
B) It degrades into amino acids
C) It fuses with the Golgi apparatus
D) It rapidly disassembles
  • 4. Which proteins are responsible for the recruitment and self-assembly of clathrin?
A) Histone complexes
B) Microtubule motors
C) Adaptor molecules
D) Ribosomal proteins
  • 5. Which specific protein is mentioned as being used in synaptic vesicle formation?
A) AP180
B) ch-TOG
C) TACC3
D) CKAP5
  • 6. Besides recruiting clathrin, what additional function does Epsin perform?
A) Helps deform the membrane to aid budding
B) Synthesizes ATP for the budding process
C) Degrades the clathrin triskelion
D) Transports vesicles to the nucleus
  • 7. What is the shape formed by clathrin triskelions on the membrane?
A) A spherical droplet
B) A polyhedral lattice
C) A hollow cylinder
D) A linear filament
  • 8. To what does the clathrin triskelion foot bind?
A) The cell membrane directly
B) Adaptor proteins
C) Lipid bilayer phospholipids
D) Microtubules
  • 9. What happens to clathrin-coated vesicle formation during mitosis?
A) It continues at a constant rate
B) It increases significantly
C) It occurs only in the nucleus
D) It is shut down
  • 10. Which two proteins form a complex with clathrin during mitosis?
A) Actin and Myosin
B) Tubulin and Dynein
C) AP180 and Epsin
D) TACC3 and ch-TOG/CKAP5
  • 11. What is the function of the TACC3/ch-TOG/clathrin complex during mitosis?
A) Creating the cell wall
B) Stabilizing kinetochore fibers
C) Regulating cytoplasmic pH
D) Inhibiting DNA replication
  • 12. What is required for the clathrin complex to crosslink microtubules?
A) The presence of Pitstop 2
B) The disassembly of the polyhedral lattice
C) Its trimeric structure
D) Its binding to the Golgi membrane
  • 13. What are Pitstop 1 and Pitstop 2?
A) Microtubule stabilizers
B) Selective clathrin inhibitors
C) Growth factors
D) Adaptor proteins
  • 14. How do Pitstop compounds interfere with cellular invasion?
A) By binding directly to the membrane lipids
B) By degrading the spindle apparatus
C) By preventing the synthesis of AP180
D) By blocking endocytic ligand association with the clathrin terminal domain
  • 15. Which molecule is used to create the microtubule binding surface in the mitotic spindle?
A) The polyhedral lattice and AP180
B) The amino-terminal domain of clathrin heavy chain and the TACC domain of TACC3
C) The ch-TOG terminal domain only
D) The membrane-bound adaptor proteins
  • 16. Who first isolated clathrin in 1976?
A) Charles Darwin
B) Barbara Pearse
C) Robert Hooke
D) Graeme Mitchison
  • 17. What is the shape of a clathrin protein unit?
A) Sphere
B) Cylinder
C) Triskelion
D) Cube
  • 18. Who suggested the name 'clathrin' to Barbara Pearse?
A) Gregor Mendel
B) Louis Pasteur
C) Isaac Newton
D) Graeme Mitchison
  • 19. How many heavy chains are in one clathrin triskelion?
A) Six
B) Two
C) Three
D) Four
  • 20. What is the approximate molecular weight of a clathrin heavy chain?
A) 190 kDa
B) 100 kDa
C) 25 kDa
D) 500 kDa
  • 21. What is the approximate molecular weight of a clathrin light chain?
A) 10 kDa
B) 25 kDa
C) 80 kDa
D) 190 kDa
  • 22. On which human chromosome is the main clathrin heavy chain gene located?
A) Chromosome 22
B) Chromosome 17
C) Chromosome 13
D) Chromosome 1
  • 23. Where is the second clathrin heavy chain gene primarily expressed?
A) Brain
B) Muscle
C) Liver
D) Skin
  • 24. What structure does the N-terminal domain of the clathrin heavy chain form?
A) Random coil
B) Beta-sheet sandwich
C) Seven-bladed beta-propeller
D) Alpha helix bundle
  • 25. To enclose a vesicle, how many pentagons must be present in the lattice?
A) 6
B) 36
C) 12
D) 24
  • 26. What is the smallest clathrin cage commonly imaged called?
A) Micro-cage
B) Triskelion-shell
C) Nano-sphere
D) Mini-coat
  • 27. How many hexagons are in a mini-coat?
A) Twelve
B) Zero
C) Two
D) Five
  • 28. What type of rings are necessary for curved lattice formation?
A) Triangles
B) Pentagons
C) Squares
D) Hexagons
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