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How to use a microscope
Contributed by: Simpson
  • 1. What is the primary function of a microscope?
A) To dissect specimens
B) To illuminate a room
C) To magnify small objects
D) To measure distances
  • 2. Which part of the microscope focuses light onto the specimen?
A) Stage
B) Eyepiece
C) Objective lens
D) Condenser
  • 3. What is the name of the lens you look through?
A) Objective lens
B) Diaphragm
C) Condenser lens
D) Eyepiece
  • 4. Which adjustment knob is used for coarse focusing?
A) Condenser adjustment knob
B) Fine adjustment knob
C) Coarse adjustment knob
D) Stage adjustment knob
  • 5. What is the purpose of the stage clips?
A) To focus the image
B) To move the stage
C) To hold the slide in place
D) To adjust the light intensity
  • 6. Which part controls the amount of light passing through the specimen?
A) Diaphragm
B) Objective lens
C) Coarse focus knob
D) Eyepiece
  • 7. What does 'parfocal' mean in relation to microscope objective lenses?
A) The lenses are all made of glass
B) The magnification is consistent
C) The image is always perfectly clear
D) The image stays in focus when changing objectives
  • 8. How should you carry a microscope?
A) By the objective lenses
B) By the stage
C) By the eyepiece
D) With one hand on the arm and the other under the base
  • 9. What should you use to clean the microscope lenses?
A) Facial tissue
B) Paper towel
C) Cloth
D) Lens paper
  • 10. When using the highest power objective, what adjustment knob should you use?
A) Condenser adjustment knob
B) Stage adjustment knob
C) Fine adjustment knob
D) Coarse adjustment knob
  • 11. What is the total magnification when using a 10x eyepiece and a 40x objective?
A) 400x
B) 1040x
C) 4x
D) 50x
  • 12. What is the first step in preparing a wet mount slide?
A) Clean the slide
B) Place a drop of liquid on the slide
C) Place the coverslip on the slide
D) Add stain to the specimen
  • 13. What is the purpose of a coverslip?
A) To flatten the specimen and protect the objective lens
B) To hold the slide in place
C) To add contrast to the specimen
D) To magnify the specimen
  • 14. Which type of microscope is used to observe living cells in their natural state?
A) Phase contrast microscope
B) Scanning electron microscope
C) Transmission electron microscope
D) Compound microscope
  • 15. What is the purpose of oil immersion?
A) To reduce the amount of light needed
B) To clean the objective lens
C) To increase resolution at high magnification
D) To stain the specimen
  • 16. Before storing the microscope, what objective should be in place?
A) Highest power objective
B) Lowest power objective
C) Any objective
D) Oil immersion objective
  • 17. What does resolution refer to?
A) The magnification power of the microscope
B) The ability to distinguish between two closely spaced objects
C) The brightness of the image
D) The depth of focus
  • 18. What should you do if you can't see anything when looking through the microscope?
A) Clean the eyepiece
B) Check the light source and objective lens position
C) Increase the magnification
D) Add more stain
  • 19. What is the function of the arm of the microscope?
A) Controls the light source
B) Supports the stage
C) Supports the body tube and is used for carrying
D) Holds the objective lenses
  • 20. The base of the microscope...
A) Holds the objective lenses
B) Provides a stable platform for the microscope
C) Contains the light source
D) Adjusts the focus
  • 21. What is the field of view?
A) The visible area seen through the eyepiece
B) The magnification power
C) The brightness of the light source
D) The objective lens in use
  • 22. What is the working distance?
A) The field of view diameter
B) The distance between the eyepieces
C) The distance between the objective lens and the specimen
D) The length of the microscope arm
  • 23. Why is it important to center the specimen before increasing magnification?
A) To improve the contrast
B) To increase the resolution
C) To prevent the specimen from moving out of the field of view
D) To reduce the amount of light needed
  • 24. What is the refractive index?
A) The strength of the objective lens
B) The amount of light produced by the light source
C) A measure of how much light bends when it passes from one medium to another
D) The size of the specimen being observed
  • 25. What is the role of immersion oil in microscopy?
A) To enhance the color of the specimen
B) To act as a mounting medium for the specimen
C) To clean the objective lens
D) To minimize light refraction and improve resolution
  • 26. What is the numerical aperture (NA) of a microscope objective?
A) The distance between the lens and the specimen
B) A measure of the lens' ability to gather light and resolve fine specimen detail
C) The field of view diameter
D) The magnification power of the lens
  • 27. What is Köhler illumination?
A) A method for cleaning microscope lenses
B) A technique for optimizing the light path in a microscope to produce a high-quality image
C) A type of stain used in microscopy
D) A type of microscope objective
  • 28. What type of illumination is used in a darkfield microscope?
A) Direct light passes through the specimen
B) Light is scattered by the specimen and enters the objective lens
C) Polarized light is used to illuminate the specimen
D) Fluorescent light is used to illuminate the specimen
  • 29. What is fluorescence microscopy used for?
A) To observe the surface topography of a specimen
B) To visualize specific structures or molecules within a cell or tissue
C) To measure the electrical properties of a cell
D) To perform microsurgery
  • 30. What is a microtome used for?
A) To measure the size of cells
B) To cut thin sections of tissue for microscopy
C) To mix reagents for staining
D) To sterilize microscope slides
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