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Gut microbiota - Quiz
Contributed by: Neal
  • 1. What dietary component is often lacking in the Western-pattern diet that gut flora need to flourish?
A) Cholesterol
B) Simple sugars
C) Saturated fats
D) Fiber
  • 2. How does the Western-pattern diet affect energy generation by gut flora?
A) It decreases the amount of energy generated
B) It has no effect on energy generation
C) It increases the amount of energy generated
D) It stops energy generation entirely
  • 3. What is observed when gut microbiome diversity is high?
A) Microbiota manipulate the host more frequently
B) Microbiota stop functioning
C) Microbiota spend energy competing with each other
D) Microbiota work together to influence host cravings
  • 4. Which organ plays a dominant role in blood glucose homeostasis?
A) Small intestine
B) Stomach
C) Liver
D) Pancreas
  • 5. What happens when lipids are administered directly into the upper intestine?
A) Lipid absorption is inhibited
B) Glucose production is suppressed
C) Glucose production is increased
D) Insulin production is stopped
  • 6. What is the result of cutting the neural connection between the brain and the gut regarding intestinal lipids?
A) It causes immediate weight gain
B) It enhances the ability of lipids to inhibit glucose production
C) It blocks the ability of lipids to inhibit glucose production
D) It has no effect on glucose production
  • 7. How has human gut biota diversity changed since the evolutionary split from Pan?
A) It has remained identical
B) It has increased
C) It has decreased
D) It has doubled
  • 8. Which bacterial phylum has increased in humans compared to other great apes?
A) Methanobrevibacter
B) Lachnospiraceae
C) Bacteroidetes
D) Fibrobacter
  • 9. What role does the gut microbiota play in termites?
A) Production of complex vitamins
B) Prevention of viral infections
C) Regulation of body temperature
D) Digestion of lignocellulose
  • 10. What happens when termite gut microbiota are disrupted by antibiotics?
A) Termites lose their host-specific traits
B) Termites live longer
C) Digestive function becomes more efficient
D) Digestive function is severely damaged
  • 11. How do gut microbiota assemble in cockroaches?
A) In a deterministic fashion
B) Based solely on age
C) Based on environmental temperature
D) In a random fashion
  • 12. What effect do subtherapeutic doses of antibiotics have on the ratio of Firmicutes and Lachnospiraceae in mice?
A) It is significantly reduced
B) It disappears completely
C) It remains unchanged
D) It is significantly elevated
  • 13. What was the conclusion regarding caloric extraction after antibiotic treatment in mice?
A) Increased capacity to extract calories from indigestible constituents
B) Inability to process fats
C) Decreased capacity to extract calories
D) No change in caloric extraction
  • 14. Besides diet, what other factor may play a role in the selection of bacterial lineages in insects?
A) Atmospheric pressure
B) Inoculum density
C) Host phylogeny
D) Solar radiation
  • 15. What does dieting (restricting calorie intake) do to gut flora?
A) It eliminates the gut flora
B) It drives changes to their composition
C) It prevents the gut flora from evolving
D) It forces the gut flora into a dormant state
  • 16. What factor does NOT dictate the classification of enterotypes?
A) Diet
B) Microbiome composition
C) Gender
D) Bacteriological ecosystem
  • 17. Which bacteria becomes the dominant species in the stomach when present?
A) Veillonella
B) Streptococcus
C) Rothia
D) H. pylori
  • 18. How many phyla of bacteria represent the main gastric microbiota?
A) Three
B) Ten
C) Five
D) Two
  • 19. What condition in the distal small intestine supports Enterobacteriaceae?
A) High acidity
B) Alkaline conditions
C) High oxygen levels
D) Extreme heat
  • 20. What is the consequence of bacterial overgrowth in the small intestine?
A) Intestinal failure
B) Weight loss
C) Increased nutrient absorption
D) Improved immune function
  • 21. Which organisms make up 99% of the large intestine and feces flora?
A) Aerobic bacteria
B) Obligate anaerobes
C) Viruses
D) Fungi
  • 22. What percentage of fecal nitrogen is accounted for by bacteria?
A) 60%
B) 99%
C) 30%
D) 10%
  • 23. What technique is used to identify bacteria in fecal specimens?
A) Blood sampling
B) 16S rRNA gene sequences
C) Gastric analysis
D) Biopsy extraction
  • 24. Which two phyla constitute 90% of the intestinal microbiota?
A) Firmicutes and Actinobacteria
B) Bacteroidota and Bacillota
C) Verrucomicrobiota and Bacteroidota
D) Actinomycetota and Pseudomonadota
  • 25. What is the most common bacterial species found in healthy adults?
A) Bacteroides
B) Bifidobacterium
C) Helicobacter pylori
D) Faecalibacterium prausnitzii
  • 26. What type of relationship exists between humans and gut flora?
A) Antagonistic
B) Parasitic
C) Mutualistic, symbiotic
D) Commensal only
  • 27. Which vitamins are produced by gut microorganisms for the host?
A) Vitamin A and E
B) Biotin and vitamin K
C) Vitamin C and D
D) B12 and calcium
  • 28. Which metabolic end products help train the immune system?
A) Propionate and acetate
B) Glucose and lipids
C) Biotin and niacin
D) Proteins and amino acids
  • 29. What is associated with extensive modification and imbalances of the gut microbiota?
A) Obesity
B) Muscle growth
C) Improved vision
D) Increased height
  • 30. What factors can disrupt the microorganism population of the large intestine?
A) Antibiotics, stress, and parasites
B) Vegetable consumption
C) Vitamin supplements
D) Exercise, sleep, and hydration
  • 31. Which term describes the aggregate of all genomes of the gut microbiota?
A) Digestive proteome
B) Human genome
C) Microbial ecosystem
D) Gastrointestinal metagenome
  • 32. What is the primary location of the human microbiome?
A) The gut
B) The respiratory tract
C) The oral cavity
D) The skin
  • 33. Which term describes an imbalance in the gut microbiota?
A) Homeostasis
B) Symbiosis
C) Dysbiosis
D) Metabolism
  • 34. Which region of the digestive tract contains the highest microbial density?
A) The esophagus
B) The small intestine
C) The stomach
D) The colon
  • 35. What percentage of gut bacteria comes from about 30 or 40 species?
A) 99%
B) 50%
C) 75%
D) 25%
  • 36. What percentage of the dry mass of feces is estimated to be bacteria?
A) 55%
B) 30%
C) 10%
D) 85%
  • 37. What process do gut microorganisms use to convert dietary fiber into short-chain fatty acids?
A) Fermentation
B) Oxidation
C) Digestion
D) Respiration
  • 38. The gut flora functions similarly to which organ system due to its production of systemic compounds?
A) Endocrine organ
B) Lymphatic system
C) Nervous system
D) Respiratory system
  • 39. What type of diet is mentioned as stimulating the growth of beneficial bacteria for the brain?
A) High-protein diet
B) Paleo diet
C) Keto diet
D) Mediterranean diet
  • 40. What percentage of bacteria in the gut are anaerobes?
A) Over 99%
B) Less than 10%
C) Approximately 75%
D) About 50%
  • 41. Why are many gut microbial species not studied outside their host?
A) They are too small
B) They are protected by patents
C) They are strictly anaerobic
D) They cannot be cultured
  • 42. Which organization aims to better describe the human gut microbiota?
A) The Global Gut Institute
B) The International Microbiota Consortium
C) The World Health Organization
D) The Human Microbiome Project
  • 43. Which genus makes up approximately 30% of all gut bacteria?
A) Clostridium
B) Bifidobacterium
C) Lactobacillus
D) Bacteroides
  • 44. Which genus is most frequently found in individuals with inflammatory bowel disease?
A) Saccharomyces
B) Aspergillus
C) Rhodotorula
D) Candida
  • 45. Which genus is most frequently found in individuals with hepatitis B cirrhosis?
A) Candida
B) Trametes
C) Rhodotorula
D) Penicillium
  • 46. What is associated with a reduction in microbial diversity in the gut?
A) Industrialization
B) Exercise
C) High-fiber diets
D) Agricultural practices
  • 47. What did researchers propose in 2018 regarding the human microbiota?
A) A biobank repository
B) A classification overhaul
C) A global vaccination program
D) A ban on antibiotic use
  • 48. Which of the following genera is present to a lesser extent in the gut?
A) Bifidobacterium
B) Faecalibacterium
C) Lactobacillus
D) Bacteroides
  • 49. How do microbial populations change within a single individual over time?
A) They change daily
B) They fluctuate randomly
C) They stay fairly constant
D) They completely replace themselves monthly
  • 50. Which genus is currently being researched to determine if it is a permanent or transient member of the gut flora?
A) Candida
B) Bacteroidetes
C) Saccharomyces
D) Penicillium
  • 51. Which organism is known to reach the intestines after ingestion and can cause auto-brewery syndrome?
A) Saccharomyces cerevisiae
B) Candida albicans
C) Penicillium
D) Firmicutes
  • 52. How is Candida albicans believed to be acquired by humans?
A) Through environmental exposure in adulthood
B) Vertical transmission at birth
C) Through consumption of cheese
D) Through consumption of brewer's yeast
  • 53. Which viruses are the most numerous in the human virome?
A) Plant viruses
B) Bacteriophages
C) Viruses that infect native human cells
D) Fungal viruses
  • 54. The US population shows a high representation of enzymes for the degradation of which substance?
A) Cysteine
B) Glutamate
C) Polysaccharides
D) Glutamine
  • 55. Which group was found to have the highest level of bacterial diversity recorded in a human group?
A) Florence children
B) US urban population
C) Yanomami Amerindians
D) Hadza hunter-gatherers
  • 56. In the comparison between European children and children from Boulpon, which bacteria dominated the European children's microbiome?
A) Treponema
B) Prevotella
C) Firmicutes
D) Bacteroidetes
  • 57. Which taxon was found to be enriched in the Hadza hunter-gatherer community?
A) Penicillium
B) Saccharomyces
C) Treponema
D) Candida
  • 58. What is observed in the mouths and throats of malnourished children?
A) More yeast
B) Less yeast
C) No yeast
D) Only pathogenic bacteria
  • 59. How many microbe families were found to vary based on race or ethnicity by the American Gut Project and Human Microbiome Project?
A) Twelve
B) Two
C) Six
D) Twenty
  • 60. Which ancestry group had the highest level of gut microbiota diversity in the HELIUS study?
A) Surinamese
B) Dutch
C) South Asian
D) African
  • 61. What was the main limitation of the American Gut Project regarding race and ethnicity findings?
A) Inaccurate sequencing technology
B) Small sample size
C) Lack of geographical focus
D) Too many participants
  • 62. Besides intestinal health, what systemic process does gut microbiota influence?
A) Auditory processing
B) Skeletal growth
C) Visual acuity
D) Immune regulation
  • 63. What organism is mentioned as becoming established if gut flora is disrupted?
A) Lactobacillus acidophilus
B) Escherichia coli
C) Bifidobacterium
D) Clostridioides difficile
  • 64. What percentage of the American Gut Project participants were white?
A) 10%
B) 25%
C) 90%
D) 50%
  • 65. Which cells proliferate to produce the mucosa in the developing intestine?
A) B cells
B) T helper cells
C) Goblet cells
D) Stem cells
  • 66. What does GALT stand for?
A) General-associated lymphoid tissue
B) Gut-associated lymphoid tissue
C) Gut-aligned lymphatic tissue
D) Gastric-associated lymphoid tissue
  • 67. What do cytokines drive the immune system to produce to protect itself?
A) Steroids
B) Mucosa
C) Inflammation
D) Antibodies
  • 68. What do segmented filamentous bacteria drive the production of?
A) IgA antibodies
B) Anti-inflammatory cytokines
C) Inflammatory cytokines
D) Mucosa layers
  • 69. Which antibody class are B cells switched to by gut flora regulation?
A) IgA
B) IgM
C) IgE
D) IgG
  • 70. Which signaling pathway in intestinal epithelial cells causes B cell class switching to IgA?
A) Cytokine receptor signaling
B) NF-kB
C) B cell receptor signaling
D) T helper signaling
  • 71. What is the primary function of saccharolytic fermentation in the gut?
A) Turning carbohydrates into short-chain fatty acids.
B) Breaking down proteins into complex sugars.
C) Producing vitamins like biotin and folate.
D) Synthesizing chitin polymers.
  • 72. Which short-chain fatty acid provides energy specifically to gut cells?
A) Lactic acid
B) Propionic acid
C) Butyric acid
D) Acetic acid
  • 73. Which short-chain fatty acid is primarily used by muscle tissue?
A) Lactose
B) Acetic acid
C) Propionic acid
D) Butyric acid
  • 74. Which microorganism is a member of domain Archaea, not bacteria?
A) Lactobacillus
B) Escherichia coli
C) Methanobrevibacter smithii
D) Ruminococcus
  • 75. What substance found in insects can be degraded by bacterial strains in primates?
A) Glucose
B) Lactose
C) Chitin
D) Cellulose
  • 76. What term describes the interaction between drugs and the microbiome?
A) Pharmacomicrobiomics
B) Microbial metabolism
C) Xenobiotic synthesis
D) Saccharolytic fermentation
  • 77. What kind of mechanism involves microbial enzymes modifying the chemical structure of drugs?
A) Enzymatic inhibition
B) Indirect mechanism
C) Host-mediated metabolism
D) Direct mechanism
  • 78. How does the gut microbiota influence host metabolizing enzymes via the indirect pathway?
A) Through microbial metabolites
B) By direct chemical cleavage
C) By physically binding to cytochrome P450
D) Through protein synthesis inhibition
  • 79. Which enzyme is mentioned as influencing host metabolizing enzymes?
A) Dehydroxylase
B) Beta-glucuronidase
C) Decarboxylase
D) Cytochrome P450
  • 80. Which drug is mentioned as being inactivated by the gut microbiota?
A) Irinotecan
B) Digoxin
C) L-dopa
D) Lovastatin
  • 81. Which microorganism is identified as responsible for inactivating digoxin?
A) Bifidobacterium bifidum
B) Eggerthella lanta
C) Escherichia coli
D) Lactobacillus acidophilus
  • 82. What is the primary cause of gastrointestinal toxicity associated with irinotecan?
A) Microbiome-encoded beta-glucuronidase
B) Inactivation by Eggerthella lanta
C) Direct chemical modification by P450
D) Microbial decarboxylase enzymes
  • 83. What describes lovastatin in the context of the provided text?
A) A chemotherapeutic agent
B) An active acid hydroxylated drug
C) A lactone prodrug
D) A heart failure medication
  • 84. What is the function of the cytochrome-encoding operon in Eggerthella lanta?
A) Digoxin-inactivation
B) Irinotecan metabolism
C) L-dopa decarboxylation
D) Lovastatin activation
  • 85. Which enzyme is responsible for the first step of secondary bile acid metabolism?
A) Cholic acid reductase
B) Short chain fatty acids
C) Bile salt hydrolases
D) Lactobacillus dehydrogenase
  • 86. What happens to the colon when exposed to secondary bile acids resulting from dysbiosis?
A) DNA damage
B) Improved homeostasis
C) Increased germination of C. difficile
D) Reduced gut motility
  • 87. Which of the following is included in the broadly defined gut-brain axis?
A) Gall bladder
B) Liver
C) Vagus nerve
D) Pancreas
  • 88. Where are primary bile acids synthesized?
A) Small intestine
B) Hepatocytes
C) Colon
D) Stomach
  • 89. Where are primary bile acids stored?
A) Colon
B) Liver
C) Small intestine
D) Gall bladder
  • 90. What is the newborn's gut microbiota compared to during early development?
A) The father's gut microbiota.
B) A sterile environment.
C) The mother's third-trimester samples.
D) The mother's first-trimester samples.
  • 91. What do probiotics contain?
A) Live microorganisms
B) Synthesized fiber compounds
C) Dead microbial cells
D) Standardized pharmaceutical salts
  • 92. Which of the following is considered a pharmabiotic definition?
A) A probiotic that is not for human use
B) Raw, unprocessed fiber from plants
C) Genetically engineered probiotics
D) Any food that is high in sugar
  • 93. Which bacteria is listed as potentially effective for decreasing symptoms of irritable bowel syndrome?
A) Escherichia coli strain K12
B) Salmonella enterica
C) Bifidobacterium infantis
D) Helicobacter pylori
  • 94. What percentage of marketed non-antibiotic drugs were found to inhibit at least one gut bacterial strain?
A) 50%
B) 24%
C) 40%
D) 10%
  • 95. How many gut bacterial strains were tested against marketed drugs in the research study?
A) 1000
B) 24
C) 100
D) 40
  • 96. What do prebiotics provide for advantageous gut flora?
A) Genetic modification
B) Substrate for growth
C) Antibiotic protection
D) Regulatory approval
  • 97. Which axis is involved in the physiological stress that affects the gut epithelium in IBD?
A) Hypothalamic–thyroid axis
B) Hypothalamic–pituitary–adrenal axis
C) Renin–angiotensin–aldosterone axis
D) Sympathetic–parasympathetic axis
  • 98. Which of these is a symptom of Irritable Bowel Syndrome?
A) Lack of inflammation
B) Excessive weight gain
C) Abdominal pain
D) High gut diversity
  • 99. Which bacteria are found at high levels in the microbiome of individuals with Irritable Bowel Syndrome?
A) Facultative anaerobic bacteria such as Escherichia coli
B) Actinobacteria
C) Lactobacilli
D) Bifidobacteria
  • 100. Which microbial group exhibits more persistent changes after antibiotic treatment in adults?
A) Fungal communities
B) Viral communities
C) Bacterial communities
D) Protozoan communities
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