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Negative-strand RNA virus - Quiz
Contributed by: Ainsworth
  • 1. Which arthropod-transmitted virus is mentioned in the text?
A) Rift Valley fever virus
B) Rabies virus
C) Measles virus
D) Rinderpest virus
  • 2. Which animal is a common vector for the Lassa fever virus?
A) Birds
B) Bats
C) Rodents
D) Mosquitoes
  • 3. Which of these is a human-specific negative-strand RNA virus?
A) Rift Valley fever virus
B) Hantavirus
C) Ebola virus
D) Mumps virus
  • 4. What kind of animals are commonly affected by influenza viruses?
A) Only rodents
B) Insects and reptiles
C) Fish and amphibians
D) Birds and mammals
  • 5. In what year was the vesicular stomatitis virus first isolated?
A) 2018
B) 1925
C) 2000
D) 1950
  • 6. Why was the discovery of the vesicular stomatitis virus unique at the time?
A) It was a negative sense RNA virus
B) It was not transmissible to humans
C) It was the first virus ever studied
D) It was a DNA virus
  • 7. Which disease became the second to be eradicated in the early 21st century?
A) Rinderpest
B) Influenza
C) Measles
D) Rabies
  • 8. What type of virus caused smallpox, the first disease to be eradicated?
A) DNA virus
B) Retrovirus
C) Negative-strand RNA virus
D) Positive-strand RNA virus
  • 9. What method has become common in the 21st century to identify viruses in the environment?
A) Antibody titration
B) Microscopic imaging
C) Viral metagenomics
D) Cell culture isolation
  • 10. Phylogenetic analysis of which enzyme showed that negative-strand RNA viruses share a common ancestor?
A) Protease
B) DNA polymerase
C) RdRp
D) Reverse transcriptase
  • 11. In which year was the phylum Negarnaviricota established?
A) 1999
B) 1925
C) 2005
D) 2018
  • 12. Which of the following viruses is transmitted by bats?
A) Rinderpest virus
B) Rabies virus
C) Tomato spotted wilt virus
D) Lassa fever virus
  • 13. Which realm were negative-strand RNA viruses placed into after the establishment of Negarnaviricota?
A) Negarnavirales
B) Mononegavirales
C) Riboviria
D) Viridae
  • 14. What is the primary vector for the tomato spotted wilt virus mentioned in the text?
A) Birds
B) Arthropods
C) Bats
D) Rodents
  • 15. The Ebola virus is known to be transmitted by which vertebrate group?
A) Insects
B) Rodents
C) Birds
D) Bats
  • 16. What is the primary function of the viral enzyme RdRp in negative-strand RNA viruses?
A) Encasing the viral capsid
B) Translating proteins from the host genome
C) Forming the viral envelope
D) Synthesizing mRNA from the genome
  • 17. Which phylum do negative-strand RNA viruses belong to?
A) Riboviria
B) Orthornavirae
C) Haploviricotina
D) Negarnaviricota
  • 18. What is the main characteristic of the subphylum Haploviricotina?
A) Mostly non-segmented genomes
B) Lack of an RdRp enzyme
C) Ability to snatch caps from host mRNA
D) Primarily segmented genomes
  • 19. What mechanism does the RdRp of Polyploviricotina use for mRNA production?
A) Synthesizing caps on mRNA
B) Snatching caps from host mRNAs
C) Replicating without mRNA synthesis
D) Translating DNA directly
  • 20. What is the Greek origin of the prefix 'Haplo' in Haploviricotina?
A) Simple
B) Segmented
C) Double
D) Complex
  • 21. What does 'Polyplo' mean in the name Polyploviricotina?
A) Simple
B) Circular
C) Complex
D) Linear
  • 22. What is the name of the template used by RdRp to create genomic negative-sense RNA?
A) Positive-sense antigenome
B) Host messenger RNA
C) Double-stranded DNA
D) Positive-sense mRNA
  • 23. What is a characteristic of almost all negative-strand RNA virus genomes?
A) Exclusively non-segmented
B) Circular
C) Double-stranded
D) Linear
  • 24. What are the palindromic nucleotide sequences at the ends of all negative-strand RNA genomes called?
A) RNA replicase sites
B) Host-derived caps
C) Segmented ends
D) Terminal inverted repeats
  • 25. Where does RdRp bind to initiate replication of the −ssRNA genome?
A) Trailer sequence on the 5'-end
B) Leader sequence on the 3'-end
C) Gene end signal
D) Poly(A) tail
  • 26. What is synthesized at the 3'-end of the mRNA to finish transcription?
A) Polyadenylated tail
B) Hairpin loop
C) 5'-end triphosphate
D) Leader sequence
  • 27. How is the poly(A) tail constructed by RdRp?
A) Snatching from host RNA
B) Copying from the genome trailer
C) Binding to the 5'-end
D) Stuttering on a sequence of uracils
  • 28. What structure stops transcription in ambisense viruses?
A) Leader sequence
B) Hairpin loop structure
C) Poly(A) tail
D) 5'-end cap
  • 29. Which motif is found in the N-terminal lobe of the capsid proteins?
A) 5-A motif
B) 5-H motif
C) 3-H motif
D) N-terminal envelope motif
  • 30. Which family of −ssRNA viruses lacks an outer viral envelope?
A) Riboviria
B) Aspiviridae
C) Haploviricotina
D) Polyploviricotina
  • 31. What are possible shapes of a −ssRNA virion?
A) Only pleomorphic and tubular
B) Cubic, pyramidal, or hexagonal
C) Only filamentous and spherical
D) Filamentous, pleomorphic, spherical, or tubular
  • 32. When is the viral genome encapsidated?
A) Only during the transcription of mRNA
B) As new nucleotide sequences are synthesized
C) After the mRNA is released
D) Before RdRp binds the leader sequence
  • 33. What is the maximum number of segments found in −ssRNA virus genomes mentioned in the text?
A) One
B) Ten
C) Five
D) Twelve
  • 34. Which subphylum is characterized by universal genome segmentation?
A) Negarnaviricota
B) Mononegavirales
C) Haploviricotina
D) Polyploviricotina
  • 35. Which viruses are identified as the likely sister clade of −ssRNA viruses?
A) Reoviruses
B) Retroviruses
C) Coronaviruses
D) Adenoviruses
  • 36. To which kingdom does the phylum Negarnaviricota belong?
A) Pararnavirae
B) Orthornavirae
C) Haploviricotina
D) Riboviria
  • 37. Which Baltimore classification group contains −ssRNA viruses?
A) Group VI
B) Group III
C) Group V
D) Group IV
  • 38. How many total classes are contained within the two subphyla of Negarnaviricota?
A) Four
B) Six
C) Eight
D) Ten
  • 39. Which subphylum typically has nonsegmented genomes?
A) Polyploviricotina
B) Insthoviricetes
C) Haploviricotina
D) Bunyaviricetes
  • 40. Which class contains the order Muvirales?
A) Milneviricetes
B) Monjiviricetes
C) Chunquiviricetes
D) Yunchangviricetes
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