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Supercritical Fluid Chromatography
Contributed by: Porter
  • 1. Which of the following is commonly used as the mobile phase in SFC?
A) Water
B) Methanol
C) Carbon dioxide
D) Acetone
  • 2. What state is the mobile phase in SFC?
A) Solid
B) Liquid
C) Gas
D) Supercritical
  • 3. What is the mechanism of separation in SFC based on?
A) Differences in boiling point
B) Differences in solubility in the mobile phase
C) Differences in molecular weight
D) Differences in conductivity
  • 4. What is the purpose of using a back pressure regulator in SFC?
A) Maintain supercritical conditions
B) Increase column temperature
C) Enhance detector sensitivity
D) Avoid solvent contamination
  • 5. What advantage does SFC offer over traditional HPLC?
A) Faster separation
B) Limited application range
C) Lower equipment cost
D) Higher resolution
  • 6. What role does co-solvent play in SFC separations?
A) Improves stationary phase selectivity
B) Reduces detector interference
C) Enhances analyte solubility
D) Stabilizes column efficiency
  • 7. Which term describes the region where the mobile phase in SFC exhibits properties of both a liquid and a gas?
A) Supercritical zone
B) Critical point
C) Transition zone
D) Near-critical region
  • 8. At supercritical conditions, the density of carbon dioxide is similar to that of which phase?
A) Liquid
B) Aqueous
C) Gas
D) Solid
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