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Plant virus - Quiz
Contributed by: Lake
  • 1. What percentage of the time does the host ribosome terminate at the leaky stop codon in TMV?
A) 95%
B) 5%
C) 100%
D) 50%
  • 2. What is the function of the extra polypeptide produced via readthrough in TMV?
A) Movement protein
B) Coat protein
C) RNA polymerase
D) Proteinase
  • 3. What is the first protein translated in viruses that use sub-genomic RNAs?
A) Proteinase
B) Coat protein
C) Replicase
D) Movement protein
  • 4. Which family of plant viruses is mentioned as having a multipartite genome?
A) Tymoviridae
B) Bromoviridae
C) Caulimoviridae
D) Potyviridae
  • 5. How many particles are required for the infection of Brome mosaic virus?
A) 5
B) 3
C) 2
D) 1
  • 6. What percentage of plant viruses adopt polyprotein processing?
A) 45%
B) 15%
C) 95%
D) 5%
  • 7. What enzyme class is responsible for cleaving polyproteins into mature proteins?
A) Lipase
B) Amylase
C) Replicase
D) Proteinase
  • 8. Besides the infection process, what is viral replicase necessary for?
A) Vector attraction
B) Symptom suppression
C) Genome packaging
D) Cell wall degradation
  • 9. How many RNAs make up the genome of the Beet necrotic yellow vein virus (BNYVV)?
A) 5
B) 1
C) 3
D) 6
  • 10. In BNYVV, what protein does RNA 1 encode?
A) P25
B) P31
C) P237
D) P26
  • 11. What protein is responsible for vector transmission in BNYVV?
A) P25
B) P14
C) P31
D) P21
  • 12. Which virus was used in the 17th-century 'tulip mania' to change flower colors?
A) Tobacco mosaic virus
B) Cauliflower mosaic virus
C) Tulip breaking virus
D) Brome mosaic virus
  • 13. Which promoter from the cauliflower mosaic virus is frequently used in plant transformations?
A) P237 promoter
B) 17S promoter
C) 35S promoter
D) P21 promoter
  • 14. Which of the following is a TMV-based expression technology?
A) Bromoviridae kit
B) BNYVV-express
C) Tulip breaking vector
D) magnICON
  • 15. What is the function of the protein P25 encoded by RNA 3 of BNYVV?
A) Symptom expression
B) Coat protein
C) Vector transmission
D) Protein cleavage
  • 16. Which viruses are generally transmitted via sap?
A) TMV, potato viruses and cucumber mosaic viruses
B) Rhabdoviridae
C) Tobacco ringspot virus
D) Beet necrotic yellow vein virus
  • 17. What common vector is often used for plant virus transmission?
A) Bacteria
B) Leafhoppers
C) Fungi
D) Zoosporic protozoa
  • 18. What determines the host range of many plant viruses?
A) The soil composition
B) The plant's height
C) The type of root system
D) The chosen insect vector
  • 19. Where do non-persistent viruses attach on the insect?
A) The haemolymph
B) The salivary glands
C) The distal tip of the stylet
D) The foregut
  • 20. Which virus class has been proposed as originally being insect viruses?
A) Tobacco rattle virus
B) Beet necrotic yellow vein virus
C) Rhabdoviridae
D) Tobacco ringspot virus
  • 21. In semi-persistent transmission, where does the virus enter?
A) The foregut of the insect
B) The salivary glands
C) The haemolymph
D) The stylet tip
  • 22. What is the name of the protein that protects circulative viruses inside aphids?
A) Symbionin
B) Coat protein
C) Helper component
D) Lipid envelope
  • 23. What characterizes propagative persistent viruses?
A) They do not replicate in insects
B) They are only found in nematodes
C) They only replicate in the plant root
D) They replicate in both the plant and the insect
  • 24. What is the function of the helper component in non-persistent and semi-persistent viruses?
A) Binding to the coat protein and insect mouthparts
B) Protecting the virus from symbionin
C) Creating a lipid envelope
D) Replicating inside the insect cell
  • 25. How do soil-borne nematodes acquire and transmit viruses?
A) Attaching to leaves
B) Feeding on infected roots
C) Replicating within the nematode gut
D) Infecting the insect vector
  • 26. Are viruses able to replicate in nematodes?
A) No evidence exists
B) Only circulative ones
C) Only propagative ones
D) Yes, always
  • 27. What do plasmodiophorids create to help other viruses enter the plant?
A) Wounds in the root
B) A protein bridge
C) A lipid envelope
D) A stylet attachment
  • 28. What is unique about the lipid coat in TSWV?
A) It allows replication in nematodes
B) It surrounds the proteins and is not seen in other classes
C) It is produced by bacterial symbionts
D) It is used for non-persistent transmission
  • 29. How are viruses classified based on transmission?
A) Propagative, circulative, and dormant
B) Non-persistent, semi-persistent, and persistent
C) Sap, insect, and nematode-borne
D) Root, leaf, and stem-borne
  • 30. What kind of microscope is required to observe plant viruses?
A) Electron microscope
B) Light microscope
C) Phase-contrast microscope
D) Fluorescence microscope
  • 31. How do viral particles assemble?
A) Through enzymatic mediation
B) Through external heating
C) Spontaneously
D) Via host ribosomes
  • 32. What is the most common shape among known plant viruses?
A) Icosahedral
B) Spherical
C) Gemini-shaped
D) Rod-shaped
  • 33. What is the typical diameter of a rod-shaped plant virus?
A) 60–80 nm
B) 15–20 nm
C) 25–50 nm
D) 5–10 nm
  • 34. What is the second most common structure for plant viruses?
A) Isometric particles
B) Filamentous particles
C) Geminiviridae particles
D) Rod-shaped particles
  • 35. What is the diameter range of isometric plant virus particles?
A) 100–200 nm
B) 25–50 nm
C) 15–20 nm
D) 300–500 nm
  • 36. How many T subunits make up the basic structure of isometric viruses with a single coat protein?
A) 15 T
B) 100 T
C) 30 T
D) 60 T
  • 37. What shape is formed when two coat proteins associate in certain plant viruses?
A) Spiral
B) Rod-shaped
C) Icosahedral
D) Cubical
  • 38. What is the characteristic structure of Geminiviridae?
A) A large sphere with a tail
B) Two isometric particles stuck together
C) A triple-layered disc
D) A single long rod
  • 39. What process results in the acquisition of a lipid envelope by a plant virus?
A) Budding off from the cell
B) Genome replication
C) Spontaneous disc stacking
D) Protein subunit assembly
  • 40. What kind of parasites are plant viruses?
A) Symbiotic microorganisms
B) Autotrophic organisms
C) Obligate intracellular parasites
D) Facultative extracellular parasites
  • 41. What is the preferred path for plant viruses to move between cells?
A) Xylem vessels
B) Root hairs
C) Stomata
D) Plasmodesmata
  • 42. What do plants use as a defense against viral infection in response to dsRNA?
A) T-cells
B) Cellular lysosomes
C) siRNA
D) Antibodies
  • 43. Who first demonstrated that sap from mosaic tobacco leaves could transmit the disease?
A) Wendell Stanley
B) A. Mayer
C) Martinus Beijerinck
D) F.O. Holmes
  • 44. What term did Martinus Beijerinck use to describe the infectious filtrate?
A) Contagium vivum fluidum
B) Filterable bacteria
C) Obligate parasite
D) Microbial toxin
  • 45. Who received the Nobel Prize in Chemistry in 1946 for his work on TMV?
A) F.O. Holmes
B) Wendell Stanley
C) Martinus Beijerinck
D) A. Mayer
  • 46. How are plant viruses typically transmitted between plants?
A) Through airborne transmission only
B) Through self-locomotion
C) Via direct sexual reproduction
D) By vectors like insects
  • 47. Which of these plant families is mentioned as susceptible to seed transmission?
A) Leguminosae
B) Orchidaceae
C) Pinaceae
D) Cactaceae
  • 48. What do plant viruses induce plant cells to produce to traffic virions?
A) Extra chlorophyll
B) Toxic proteins
C) Membranous structures
D) Thick cell walls
  • 49. Which model was used to study the lipid alterations caused by plant viruses?
A) Bacterial cultures
B) Human lung cells
C) Yeast models
D) Computer simulations only
  • 50. What percentage of plant viruses have single-stranded RNA genomes?
A) 5%
B) 75%
C) 10%
D) 65%
  • 51. What percentage of plant viruses have +ssRNA genomes?
A) 10%
B) 75%
C) 65%
D) 5%
  • 52. How many proteins are typically encoded by a plant virus genome?
A) 50-100
B) 1-3
C) 4-10
D) 15-20
  • 53. What is the typical composition of a 5' Cap structure?
A) 7MeGpppU
B) 7MeGpppT
C) 7MeGpppN
D) 7MeGpppC
  • 54. Which enzyme activity is encoded by viral proteins to allow for 5' capping?
A) Methyltransferase
B) Reverse transcriptase
C) Endonuclease
D) Polymerase
  • 55. What do cap-snatching viruses recruit to prime transcription?
A) Host transfer RNA
B) Viral ribosomal RNA
C) Host DNA
D) Capped host mRNA
  • 56. What enzyme is used by cap-snatching viruses to cleave host mRNA?
A) Reverse transcriptase
B) Methyltransferase
C) Ligase
D) Endonuclease
  • 57. What do some plant viruses use instead of a 5' Cap for translation?
A) Cap-independent translation enhancers
B) Host initiation factors
C) A poly-A tail
D) A ribozyme
  • 58. What percentage of plant viruses have ssDNA genomes?
A) 75%
B) 17%
C) 25%
D) 3%
  • 59. What proportion of animal viruses are dsDNA?
A) Three-quarters
B) Half
C) A quarter
D) One-tenth
  • 60. What proportion of bacteriophage are dsDNA?
A) Three-quarters
B) Half
C) A quarter
D) Ten percent
  • 61. What is the identity of 'N' in the 7MeGpppN cap structure?
A) Guanine or cytosine
B) Adenine or guanine
C) Uracil or adenine
D) Cytosine or thymine
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