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Growth medium - Quiz
Contributed by: Pope
  • 1. What is a minimal medium defined as?
A) A complex medium used for human cells
B) A medium containing only amino acids
C) A defined medium with just enough ingredients to support growth
D) A medium that forbids all bacterial growth
  • 2. What is typically omitted from minimal media?
A) Amino acids
B) Carbon sources
C) Salts
D) Water
  • 3. What type of microorganisms are often grown using minimal media?
A) Wild-type
B) Mutated animal cells
C) Only Gram-negative bacteria
D) Pathogenic viruses
  • 4. What is an example of a carbon source in minimal media?
A) Neomycin
B) Tetracycline
C) Glucose
D) Ampicillin
  • 5. What do the salts in minimal media provide?
A) Magnesium, nitrogen, phosphorus, and sulfur
B) Proline and histidine
C) Antibiotics and dyes
D) Glucose and sucrose
  • 6. What are supplementary minimal media used for?
A) Killing all bacteria in a sample
B) Growing wild-type microorganisms exclusively
C) Replacing water in the growth process
D) Culturing specific lines of auxotrophic recombinants
  • 7. What is the purpose of selective media?
A) Preventing all bacterial metabolism
B) Measuring the concentration of water
C) Growing all types of bacteria equally
D) Growth of only selected microorganisms
  • 8. Which antibiotics are mentioned as agents used to create selective media?
A) Penicillin and aspirin
B) Nalidixic acid and colistin
C) Ampicillin or tetracycline
D) Gancyclovir and neomycin
  • 9. What term is used for a gene that allows a cell to grow in a selective medium?
A) Recombinant
B) Exconjugant
C) Auxotroph
D) Marker
  • 10. What is used in selective media for eukaryotic cells to select transfected cells?
A) Histidine
B) Proline
C) Neomycin
D) Glucose
  • 11. What is the exception to the rule that markers allow cell growth in selective media?
A) Tetracycline
B) Gancyclovir
C) Neomycin
D) Ampicillin
  • 12. Which substance in Eosin methylene blue makes it toxic for Gram-positive bacteria?
A) Salt
B) Glucose
C) Dyes
D) Agar
  • 13. What characteristic of YM and MEA agar deters bacterial growth?
A) High salt concentration
B) Low pH
C) Lack of water
D) High antibiotic content
  • 14. What is MacConkey agar used for?
A) Gram-negative bacteria
B) Virus isolation
C) Gram-positive bacteria
D) Fungi only
  • 15. What does HIS-selective medium lack?
A) Proline
B) Histidine
C) Glucose
D) Salts
  • 16. What is Mannitol salt agar differential for?
A) Legionella
B) Staphylococci
C) Gram-negative bacteria
D) Mannitol
  • 17. Which medium is selective for Legionella pneumophila?
A) Buffered charcoal yeast extract agar
B) Sabouraud agar
C) MacConkey agar
D) Baird-Parker agar
  • 18. What is the primary use of Baird-Parker agar?
A) Legionella pneumophila
B) Gram-positive staphylococci
C) Ectomycorrhizal fungi
D) Moulds and yeasts
  • 19. What contributes to Sabouraud agar's selectivity for fungi?
A) High salt concentration
B) Low pH and high glucose
C) Presence of antibiotics only
D) Lack of nitrogen
  • 20. What is the purpose of dichloran and rose Bengal in DRBC agar?
A) To kill all yeast cells
B) To provide nitrogen to the fungi
C) To increase the pH of the medium
D) To restrict the growth of mould colonies
  • 21. Which media are used for ectomycorrhizal fungi?
A) MMN and BAF medium
B) MacConkey and Hektoen
C) CNA and Sabouraud
D) Baird-Parker and DRBC
  • 22. What is the function of nalidixic acid and colistin in CNA agar?
A) To act as a carbon source
B) To promote the growth of mould
C) To inhibit Gram-negative organisms
D) To kill all Gram-positive bacteria
  • 23. What is a defining characteristic of a chemically defined medium?
A) It contains yeast extract
B) All chemicals used are known
C) It contains animal tissue
D) The exact composition is unknown
  • 24. Which ingredient is strictly absent from a defined medium?
A) Salts
B) Glucose
C) Water
D) Plant tissue
  • 25. What substance is required in an undefined medium alongside salts and carbon sources?
A) Plant hormones
B) Animal blood
C) Water
D) Antibiotics
  • 26. Where are bacteria typically kept in long-term laboratory settings using general culture media?
A) Natural soil samples
B) Defined synthetic environments
C) Laboratory culture collections
D) Sterile air chambers
  • 27. What is a growth medium designed to support?
A) Cell apoptosis
B) Cell desiccation
C) Cell proliferation
D) Cell mutation
  • 28. Which plant is specifically mentioned as being grown in growth media?
A) Zea mays
B) Oryza sativa
C) Physcomitrella patens
D) Arabidopsis thaliana
  • 29. What type of organisms are viruses?
A) Extracellular saprophytes
B) Autotrophic microbes
C) Facultative anaerobes
D) Obligate intracellular parasites
  • 30. Which bacterial genus is capable of decomposing agar?
A) Streptococcus
B) Escherichia
C) Bacillus
D) Staphylococcus
  • 31. What do bacteria grown in liquid cultures often form?
A) Colloidal suspensions
B) Biofilm mats
C) Crystalline structures
D) Sedimentary layers
  • 32. What must be added to animal cell media to support their growth?
A) Ammonium salts
B) Blood serum
C) Agarose
D) Glucose only
  • 33. What is the liquid used to make beer that serves as a growth medium for yeast?
A) Mash
B) Wort
C) Tincture
D) Must
  • 34. What is produced when yeast grows in wort under anaerobic conditions?
A) Lactic acid
B) Acetic acid
C) Alcohol
D) Hydrogen gas
  • 35. What term describes organisms that require specialized environments due to complex needs?
A) Opportunistic
B) Fastidious
C) Ubiquitous
D) Autotrophic
  • 36. What is the primary function of differential media?
A) Supporting fastidious organisms only
B) Preventing microbial multiplication
C) Distinguishing one microorganism type from another
D) Storing specimens for long periods
  • 37. Which of the following is an example of an indicator used in differential media?
A) Sodium chloride
B) Charcoal
C) Glycerol
D) Neutral red
  • 38. What does blood agar become in the presence of beta-hemolytic organisms?
A) Transparent
B) Brown
C) Opaque
D) Red
  • 39. What color do colonies of Streptococcus agalactiae appear on Granada medium?
A) Red
B) Transparent
C) Brown
D) Blue
  • 40. X-gal plates are used to differentiate what?
A) Lac operon mutants
B) Strict anaerobes
C) Gonococci
D) Enteric bacilli
  • 41. Transport media for anaerobes must be free of what?
A) Molecular oxygen
B) Charcoal
C) Lactose
D) Glycerol
  • 42. What do enriched media contain to support fastidious organisms?
A) Reducing agents
B) Bacterial inhibitors
C) Carbon-free buffers
D) Extra nutrients
  • 43. At what temperature range is blood treated to create chocolate agar?
A) 40–45 °C
B) 60–65 °C
C) 20–25 °C
D) 80–85 °C
  • 44. Eosin methylene blue is differential for what?
A) Beta-hemolysis
B) Anaerobic growth
C) Lactose fermentation
D) Mannitol fermentation
  • 45. Thioglycolate broth is designed for which type of organisms?
A) Enteric bacilli
B) Lac operon mutants
C) Strict anaerobes
D) Fastidious aerobes
  • 46. What should transport media maintain in specimens?
A) Viability
B) High concentration
C) Active fermentation
D) Increased metabolic rate
  • 47. What is a potential risk associated with using serum-containing media?
A) Insufficient glucose levels
B) Lack of growth factors
C) Inability to support cell growth
D) Contamination issues
  • 48. Which of the following media types is known for having a standardized formulation?
A) Animal-derived medium
B) Serum-containing media
C) Fetal bovine serum
D) Serum-free media
  • 49. Why is the choice of media important for protein expression?
A) It prevents the production of proteins
B) It determines the genetic sequence of the protein
C) It influences therapeutic characteristics like glycosylation
D) It eliminates the need for purification
  • 50. What are Plasmax and HPLM examples of?
A) Chemically undefined media
B) Media that represent physiological nutrient levels
C) Serum-containing growth media
D) Media used only for bacterial cultures
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